Answer: Plants
Explanation: Plants are all ways at the start of the food chain becuase they are the only thig that can make their own food. Herbivores eat the plants to gain energy and carnivores eat the herbivores to gain their energy.
<span>Negatively charged R-groups in the primary structure.
This is because nickel acts as a cation, therefore you would be attracting anions, in this case it would be on the proteins that would be binding to the column. Once they bind to the matrix a was buffer of high salts can elute the proteins.</span>