Question

You have a column made of nickel-agarose matrix. your protein must have ________ to use this column for affinity chromatography.

Answer 1

Negatively charged R-groups in the primary structure. This is because nickel acts as a cation, therefore you would be attracting anions, in this case it would be on the proteins that would be binding to the column. Once they bind to the matrix a was buffer of high salts can elute the proteins.