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laila [671]
3 years ago
7

You have a column made of nickel-agarose matrix. your protein must have ________ to use this column for affinity chromatography.

Biology
1 answer:
belka [17]3 years ago
3 0
<span>Negatively charged R-groups in the primary structure. This is because nickel acts as a cation, therefore you would be attracting anions, in this case it would be on the proteins that would be binding to the column. Once they bind to the matrix a was buffer of high salts can elute the proteins.</span>
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Help please!........................................................
bogdanovich [222]

Answer:

The correct order would be

  • Glucose
  • ATP→ADP
  • H₂O
  • Pyruvate
  • CO₂

Explanation:

Following reactions occur in Glycolysis and Kreb's Cycle

  1. Phosphorylation of Glucose- In the first step of glycolysis, Glucose is converted into Glucose-6-Phosphate using 1 ATP molecule by Hexokinase enzyme. One phosphate group from ATP is attached to glucose by the enzyme, thus forming ADP.
  2. Dehydration- In the ninth step of Glycolysis, each of two molecules of  2-Phosphoglycerate are converted to Phosphoenol Pyruvate, by Enolase enzyme, releasing two H₂O molecules.
  3. Formation of Pyruvate- In the last or tenth step of Glycolysis, each of two molecules of Phosphoenol Pyruvate are converted to Pyrutave using an ATP by the enzyme Pyruvate Kinase.
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4 0
3 years ago
In which of these stages is mitosis most important? A tiger cub is born. A tiger cub learns to walk. A tiger cub begins to grow.
butalik [34]

Answer:

A tiger cub begins to grow

Explanation:

because that's when they change the most

5 0
3 years ago
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Compared the Mendelian Dominance, how to incomplete dominance and codominance increase the number of phenotypes? Provide an exam
REY [17]

Answer/Explanation:

<h3>Incomplete dominance</h3>

In incomplete dominance, one allele is not entirely dominant over the other, so heterozygotes (organisms with two different alleles for the gene) show an intermediate or blended phenotype.

For example, consider flower colour.

  • If the allele for red flowers (R) was dominant over the allele for white flowers (r), then there are three possible genotypes (RR, Rr, and rr) and two possible phenotypes. (Red (RR and Rr) and white (rr)).
  • However, if the allele for red flowers (R) was incompletely dominant over the allele for white flowers (r), then there are three possible genotypes (RR, Rr, rr), and three possible phenotypes (red (RR), white (rr), and pink (Rr))
<h3>Co-dominance</h3>

In incomplete dominance, two alleles are both expressed, one is not dominant over the other. Therefore, heterozygotes (organisms with two different alleles for the gene) express both traits.

For example, consider flower patterns.

  • If the allele for spots (F) was dominant over the allele for stripes (f), then there are three possible genotypes (FF, Ff, and ff) and two possible phenotypes. (Spots (Ff and ff) and stripes (ff)).
  • However, if the allele for spots (F) was co-dominant to the allele for stripes (f), then there are three possible genotypes (FF, Ff, ff), and three possible phenotypes (spots (FF), stripes (ff), and spots and stripes (Ff))

8 0
2 years ago
How many red blood cells are destroyed and replaced by your body every second?
olga2289 [7]
<span> 2.5 million red blood cells can be destroyed every second
       
       Hope this helps! 
                 :D





</span>
7 0
3 years ago
What is the part of endoplasmic reticulum without proteins attached?
lions [1.4K]

Answer:

Regions of ER that lack bound ribosomes are called smooth endoplasmic reticulum, or smooth ER.

5 0
3 years ago
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