3 years ago

You have a column made of nickel-agarose matrix. your protein must have ________ to use this column for affinity chromatography.

1 answer:

3 0

<span>Negatively charged R-groups in the primary structure. This is because nickel acts as a cation, therefore you would be attracting anions, in this case it would be on the proteins that would be binding to the column. Once they bind to the matrix a was buffer of high salts can elute the proteins.</span>

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