Answer:
CCAGGCC
CCATCGA
GGCCATC
CAT
AGGCCAT
CATCGAG
Explanation:
Shotgun sequencing is a method used to determine the nucleotide sequence of entire chromosomes/genomes. This sequencing method consists of obtaining random DNA fragments which are subsequently classified by bioinformatic tools that ordering them according to overlapping sequences called contigs. In the whole-genome shotgun (WGS) technique, the entire genome of an organism is sequenced, being the critical factor the depth of sequencing, which refers to the quality of the sequencing reads (e.g., a depth of 20X indicates that the genome is sequenced 20 times by a sequencing machine). For the human genome, WGS became available after the completion of the Human Genome Project (HGP), which enabled the generation of a reference sequence for the whole human genome. The steps of the WGS technique are the following:
1. Preparation of isolated chromosomes
2. The DNA is sheared into small fragments
3. The DNA fragments of about 1 kilobase (1000 base pairs) are incorporated into plasmids which are cloned to render pure samples of each DNA fragment
4. The plasmid clones are sequenced by sequencing machines
5. Bioinformatic tools finally are used to link DNA fragments by their overlapping ends
Answer:
The answer is C. The GPS makes a task easier
The question is incomplete as it does not have the options which are:
- going to a tailor and having a suit made to fit you
- selecting a lottery prize-winner by means of a random drawing
- going to a shoe store and trying on shoes until you find a pair that fits
- ordering the lunch special at a restaurant without looking at the menu
- picking out a video that you haven't seen yet
Answer:
Going to a shoe store and trying on shoes until you find a pair that fits
Explanation:
Antibodies are the immunoglobulin molecules secreted by the B cells which help the body providing the immunity as they interact with the antigen and help immune system to produce a response.
The variety of the B cells are produced by the body in response to different antigens which produce different type of antibodies. This interaction of antigen and antibodies is known as the complementation and just appear like if you are going to a shop to buys a pair of shoes and tries different shoes to find the best shoe of best size.
Thus, the selected option is correct.
Answer:
The correct answer is "strengths: inexpensive, easy to culture, short life cycle, large number of offspring; weaknesses: invertebrate model, some diseases such as immunological cannot be modelled, anatomical features are very different from humans"
Explanation:
The fruit fly <em>Drosophila melanogaster</em> is one of the most used animal model for genetic and biomedical studies. There are many advantages of using Drosophila as model, including that it is very inexpensive to handle, it is easy to culture, it has a short life cycle allowing to observe the changes in phenotype very quickly and its large number of offspring allows to include several repetitions per trait in a study. However, there are some weaknesses of using Drosophila to study human biology. First, obviously the fruit fly is very different from humans, it is an invertebrate and its anatomical features are very different, which makes impossible to model some disorders such as immunological diseases.
Simple version:
First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed).
Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
