Depends on their relationship now.
Answer:
The subjects of a conquered kingdom are not used to living in freedom or standing up for themselves, and are therefore slower to take up arms than are the citizens of a republic. There are three options for the conquering prince: destroy the republic, live there, or set up a puppet government. At the end of the day, Machiavelli seems to favor the first option.
OK so basically you answered your question if he has the 40th best he will be 40th out of 600