If you do a Gram-stained on a bacteria isolated from a healthy human intestine you will find mostly Gram positive spiral cells.
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As we can see in the pedigree, both of the parents do not possess the trait, but one of the four offspring (marked with red) is expressing the trait.
Since the trait is recessive, the only way that is possible that the parents who do not possess the trait to have the offspring with the trait is that the parents are heterozygous for the trait. Meaning that both of the parents have one dominant allele and one recessive allele.
Since the trait is recessive, the only way that is possible that the parents who do not possess the trait to have the offspring with the trait is that the parents are heterozygous for the trait. Meaning that both of the parents have one dominant allele and one recessive allele.