Answer:
b. False
Explanation:
These components are not sufficient to successfully perform a PCR reaction. Deoxynucleoside triphosphates (dATP, dGTP, dCTP, dTTP) are missing so that the PCR reaction can occur perfectly.
The PCR technique allows a specific fragment of the DNA molecule to be amplified thousands of times in just a few hours. This technique revolutionized research in molecular biology because it had taken a long time for DNA amplification. From PCR it is possible to obtain enough copies of a part of DNA to detect and analyze the sequence that is the target of the study.
For a PCR reaction to be performed a solution with some components must be prepared. These components are:
- Magnesium chloride buffer (to optimize reaction and act as a cofactor for polymerase)
- Forward and reverse primers (to customize the start of the enzyme reaction)
- DNA polymerase (Enzyme required for replication of desired DNA region.)
- DNA Template (the DNA to be copied)
- PCR-grade water
- Deoxynucleoside triphosphates: dATP, dGTP, dCTP, dTTP (act as bricks in the construction of DNA molecules).
Answer:
Human activity can affect the amount of carbon in the atmosphere. When fossil fuels like oil, coal, and natural gas are burned, carbon dioxide is released into the atmosphere as waste.
Explanation:
A cow will eat grass in a field, the grass is the plant and the plant eater is the cow. (Srry if this is not what your looking for)
Answer:
The plasmid must express a gene for ampicillin resistance (the protein product of the <em>bla</em> gene codes for beta-lactamase, the protein that breaks down ampicillin). The colonies on the ampicillin plate are antibiotic resistant. This means that they have taken up the transformed plasmids expressing both the <em>bla</em> gene and the GFP gene.
Explanation:
The transformation involved the genetic modification of a plasmid to incorporate the gene encoding the green fluorescent protein (GFP) from jelly fish. GFP makes cells glow under UV light.
In genetic engineering, scientists use antibiotic resistance as markers to indicate cells that have been transformed. By incorporating an antibiotic resistance gene such as <em>bla</em> into the vector (plasmid) and then growing the cells in antibiotic media, scientists determine which colonies have taken up the plasmid. Therefore, if the cells survive, this means that they contain the plasmid with antibiotic resistance gene as well as the GFP gene.
