Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
Answer:
Apoptosis does not involve:
c. lysis of the cell
Explanation:
Apoptosis is a programmed cell death that occurs under normal physiological conditions and in a controlled manner. Normally seen in cell turnover, embryogenesis, also involved in processes of immune, nervous and endocrine systems.
The main morphological and biochemical changes seen during the apoptosis are the fragmentation of DNA by endonucleases, nuclear, chromatin and cytoplasmatic condensation, apoptotic bodies formation (membrane bound-vesicles form of cell parts) and the phagocytosis (digestion) of those bodies by the scavenger cells.
Apoptosis is regulated by cell- signaling pathways, the caspases, a family of cysteine proteases, are the ones involved in the process.
In the process there is no lysis of the cell as this could lead to a inflammatory response (just happens in necrosis) which would affect contiguous cells, and will involve immune cells. In apoptosis there is just a membrane blebbing, but it does not loss its integrity.
Answer:
Technically Mitosis and Meiosis
Explanation:
Mitosis is the spliting of <em>Body Cells.</em> Mitosis goes through 4 phases that are easy to remember as PMAT. Mitosis starts with Interphase then moves to Prophase, Metaphase, Anaphase, and Telephase. These steps also occur in Meiosis. However Meiosis goes through these step twice. If you need further explaination of mitosis and meiosis I recomend the amoeba sisters.
Hope this helps!!
That is correct. Only 3 will be lit.
Naphthol AS-D chloroacetate esterase (specific) is usually positive in <u>Granulocytic</u> cells, and alpha naphthyl acetate esterase (nonspecific)is useful for identifying blast cells of <u>monocytic</u> lineage.
- White blood cells known as granulocytes have tiny granules. Proteins are present in these granules.
- Granulocytes can be classified as neutrophils, eosinophils, or basophils. The body uses granulocytes, particularly neutrophils, to fight bacterial infections.
- A monocyte is a subtype of phagocyte and a type of white blood cell.
- The role of the monocyte-macrophage cell lineage in acute and chronic allograft immunopathology is becoming more widely acknowledged.
- It is linked to lower graft performance and survival when this lineage of cells predominates in tissue that is rejecting an allograft.
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