Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Es importante que una persona tenga estabilidad emocional, social, y economica ante una situacion de embarazo para poder darle buena vida a el ser que lleva dentro.
Explanation:
Es importante tener estabilidad mental para pensar que hacer y como cuidar al bebe, y ser positvos.
Es importante tener estabilidad social para brindarle un ambiente amistoso.
Es importante tener estabilidad economica para poder aportar a las necesidades del bebe.
The final product of Meiosis is haploid daughter cells in which each cell has 23 chromosomes. What happens in meiosis is that, the cell is divided twice and this produces four daughter cells. During this process that our sex cells are produced. So the answer for this would be option D.
The correct answer is: A. A change in a cell's genetic material.
Mutations occur in DNA as a result of mistakes during the DNA replication (when repair mechanisms don’t fix it) or as the result of environmental factors (e.g. UV light). Mutations can have positive impact, by increasing the genetic variation or can have negative effect, causing the diseases or cancer.
