Answer:
im pretty sure it is underpopulation.
Answer:
All of those answer are correct.
Answer: An object with twice the mass of another object will weigh twice as much is the statement accurately describes the relationship between mass and weight.
Hope it helps you if not sorry
Explanation: A.Mass is an objects weight multiplied by the amount of gravitational force acting upon the object
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
<h2>Frequency of allele </h2>
Explanation:
Hardy Weinberg Equilibrium is used to calculate the allelic as well as genotypic frequency
Allelic frequency of dominant and recessive allele is represented by p and q respectively whereas genotypic frequency of dominant genotype is represented by
and
respectively
Given:
H allele (p) = hairy heffalump (dominant)
h allele (q) = hairless heffalump (recessive)
36% of heffalump population is hairless represents the % of recessive genotype, hh (
) =36%
Calculation of frequency of the h allele (q) :
Frequency of genotype hh (
) will be: 36/100=0.36 or 0.6*0.6
Frequency of h allele (q) will be 0.6