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Advocard [28]
3 years ago
10

What’s number 4 and 5?

Biology
1 answer:
sladkih [1.3K]3 years ago
5 0
4 is c and 5 I think is a
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b. False

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These components are not sufficient to successfully perform a PCR reaction. Deoxynucleoside triphosphates (dATP, dGTP, dCTP, dTTP) are missing so that the PCR reaction can occur perfectly.

The PCR technique allows a specific fragment of the DNA molecule to be amplified thousands of times in just a few hours. This technique revolutionized research in molecular biology because it had taken a long time for DNA amplification. From PCR it is possible to obtain enough copies of a part of DNA to detect and analyze the sequence that is the target of the study.

For a PCR reaction to be performed a solution with some components must be prepared. These components are:

  • Magnesium chloride buffer (to optimize reaction and act as a cofactor for polymerase)
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  • Deoxynucleoside triphosphates: dATP, dGTP, dCTP, dTTP (act as bricks in the construction of DNA molecules).
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