I believe the answer is UNAIDS. hope this helps!
Answer:
Problems with transfection efficiency or absence of essential mRNA modifications (capping and poly-A tail)
Explanation:
Translation is the process of protein synthesis from RNA in which the genetic information encoded in a messenger RNA (mRNA) molecule is translated into a protein sequence composed of amino acids. Translation has three steps: initiation, elongation, and termination. During translation, different elements are required to initiate, continue and complete the process, which include essential molecules such as amino acids, mRNA, tRNAs, ribosomes, energy-carrying molecules (i.e., ATP, GTP), initiation factors (i.e., eIF1A, eIF3, etc) and elongation factors (eEF-2, EIF5A). For example, humans can't synthesize nine essential amino acids (i.e., threonine, tryptophan, phenylalanine, valine, methionine, leucine, histidine, isoleucine, lysine), thereby it is imperative to include these amino acids to synthesize proteins composed of them. On the other hand, it is also fundamental to take into account that the efficiency of transfection of the molecules required during translation, as well as the absence of modifications in the mature mRNA sequence, may alter the process in vitro.
Metabolism
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Prokaryotic cells lack a nucleus.
