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Eukaryotes are organisms whose cells have a nucleus enclosed within a nuclear envelope. Eukaryotes belong to the domain Eukaryota or Eukarya; their name comes from the Greek εὖ and κάρυον. The domain Eukaryota makes up one of the domains of life in the three-domain system; the two other domains are Bacteria and Archaea. Eukaryotes represent a tiny minority of the number of living organisms; however, due to their generally much larger size, their collective worldwide biomass is estimated to be about equal to that of prokaryotes. Eukaryotes evolved approximately 1.6–2.1 billion years ago, during the Proterozoic eon.
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Cytochrome c is an enzyme found in bacteria, fungi, plants and animals. Here it is given that the human amino acid sequence for cytochrome c differs at 12 locations from the sequence in horses and pigeons. This information might be useful for inferring in preliminary stages that horses and pigeons are closely related to each other since they both show similar amount of difference from humans.
However, this hypothesis is being made with respect to the the comparison between "humans and horses" and "humans and pigeons". To further test this hypothesis pigeons and horses should be directly compared to each other without the use of a third species like humans. Hence, the sequence for cytochrome c in horse and pigeon should be compared with each other rather than comparing to humans.
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3
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If a HYPOTHESIS is wrong scientists come up with a conclusion, the hypothesis doesn't matter for the result. It is just an educated guess based on the results they expect our hope the achieve.
