1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Kamila [148]
3 years ago
12

Describe how restriction enzymes and ligase enzymes are used to make the plasmids that are introduced to bacteria cells during t

ransformation. Include the words sticky ends and complementary in your response.
Biology
1 answer:
balandron [24]3 years ago
8 0

Answer:

DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments

Explanation:

Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.

You might be interested in
Point at which the optic nerve leaves the eye, creating inability to see due to no receptor cells are located there
Iteru [2.4K]
Hi this would be called the blind spot. Hope this helps.
6 0
3 years ago
2. What type of beef cattle operation is it where producers focus on genetic improvements
Vinil7 [7]

Answer: the answer is seedstock

4 0
3 years ago
Which of the following is related to the likelihood that a mother develops postpartum depression?
Veronika [31]

Answer:

Option D

Explanation:

Postpartum depression is majorly experienced in woman who has recently entered into motherhood. This form of depression arises because of the complications involved in a child birth. It is strongly driven by sudden changes that come up in the life of a woman after becoming mother such as – loss of job, responsibility of child, tight life schedule etc. some common symptoms of this problem include – mood swing, anxiety, sleeplessness, crying etc.  

Hence, option D is correct

4 0
3 years ago
Read 2 more answers
When bacteria are present in the human body, the individual will be come sick.
Leto [7]

Answer:

well it is ture

Explanation:

ture idk

6 0
3 years ago
Read 2 more answers
From fossil evidence, you know two species shared a common ancestor approximately 50 million years ago. In comparing a known neu
MissTica

Answer:

Option c (2\times 10^{-9}) is the correct alternative.

Explanation:

Given:

Common ancestor,

= 50 million years ago

Base differences,

= 92

Now,

The predicted mutation rate will be:

= \frac{92}{50\times 10^6\times 1000}

= \frac{1.84}{10^9}

= 1.84\times 10^{-9}

or,

= 2\times 10^{-9}

Thus the above solution is the right one.

4 0
3 years ago
Other questions:
  • Which of these best represents a fatty acid molecule
    13·1 answer
  • Which of the following is true about epithelia? Which of the following is true about epithelia? Pseudostratified epithelia are c
    13·1 answer
  • Complete paralysis of the diaphragm occurs if the spinal cord is crushed or destroyed just below the c5 region.
    10·1 answer
  • Which of the following diseases is not explained by Germ theory
    12·2 answers
  • Which of the following is an example of potential energy?
    14·1 answer
  • Assignment: The West Nile Virus Exploration
    5·1 answer
  • What are the limitations of the bleeding model of genetics
    11·1 answer
  • If a gene has a proportion of 25% adenine, what is the proportion of thymine in that gene
    9·1 answer
  • Pollen is produced in structure H what is the structure called?
    10·2 answers
  • Which is true of glycolysis? (Select all that apply)
    6·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!