Irritation Threshold is defined as the name applies. It is the threshold or maximum point at which an individual has become irritated or annoyed.
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im so sorry this is so late but i hope i can still help.... its the 3rd one
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you can say either of them, but most people usually just say hola and then that's when they say como estas
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have curved protofilaments at their plus ends
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Microtubules are polymers of tubulin proteins that function as the cytoskeleton of eukaryotic cells. Microtubules are dynamic structures that can grow and shrink at a rapid rate. During this process, tubulin subunits can associate and dissociate at the plus end of the protofilament. Tubulin subunits bind to two GTP molecules, one of which is hydrolyzed to GDP after assembly. When microtubules are unstable, protofilaments curl outwards because GDP-bound tubulin has a weak affinity (thereby curving it) and disassemble. The dynamic stability of microtubules is regulated by a feedback loop: when microtubules shrink, free tubulin concentration increases and microtubules start to grow. As microtubules grow, free tubulin concentration decreases and the rate of GTP-tubulin addition also decreases.
