Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
Answer:
Explanation:
A convergent boundary (also known as a destructive boundary) is an area on Earth where two or more lithospheric plates collide. One plate eventually slides beneath the other, a process known as subduction. The subduction zone can be defined by a plane where many earthquakes occur, called the Wadati–Benioff zone.[1] These collisions happen on scales of millions to tens of millions of years and can lead to volcanism, earthquakes, orogenesis, destruction of lithosphere, and deformation. Convergent boundaries occur between oceanic-oceanic lithosphere, oceanic-continental lithosphere, and continental-continental lithosphere. The geologic features related to convergent boundaries vary depending on crust types.
Plate tectonics is driven by convection cells in the mantle. Convection cells are the result of heat generated by radioactive decay of elements in the mantle escaping to the surface and the return of cool materials from the surface to the mantle.[2] These convection cells bring hot mantle material to the surface along spreading centers creating new crust. As this new crust is pushed away from the spreading center by the formation of newer crust, it cools, thins, and becomes denser. Subduction begins when this dense crust converges with less dense crust. The force of gravity helps drive the subducting slab into the mantle.[3] As the relatively cool subducting slab sinks deeper into the mantle, it is heated, causing hydrous minerals to break down. This releases water into the hotter asthenosphere, which leads to partial melting of asthenosphere and volcanism. Both dehydration and partial melting occurs along the 1,000 °C (1,830 °F) isotherm, generally at depths of 65 to 130 km (40 to 81 mi).[4][5]
Some lithospheric plates consist of both continental and oceanic lithosphere. In some instances, initial convergence with another plate will destroy oceanic lithosphere, leading to convergence of two continental plates. Neither continental plate will subduct. It is likely that the plate may break along the boundary of continental and oceanic crust. Seismic tomography reveals pieces of lithosphere that have broken off during convergence
Using the law of superposition
The second row of values in plant experiment most likely to have "high oxygen level and carbon dioxide level would be the same".
Explanation:
The procedure by which living things convert oxygen and glucose to water and carbon, accordingly yielding vitality is called photosynthesis. It does not require the nearness of daylight and is continually happening in living life forms. Cell breath occurs in the mitochondria of cells.
Through these procedures, plants get the carbon dioxide they need, and living life forms get the oxygen they need. They are additionally crucial to the vitality trade that living things need to endure.
