Answer:
By way of introduction, A260/A280 ratio is use to measure Protein Contamination. This procedure was first described to measure protein purity in the presence of nucleic acids. However it is now commonly used to assess protein contamination of DNA. The method to determine the concentration of protein contamination by using A260/280 ratio method is well explained below by filling in the missing words.
Explanation:
The A260/A280 ratio method estimates protein __purity___ by measuring the absorbance maximum at 280nm caused by the amino acids__cytosine___, ____Adenine____, and ___Guanine_________ Since ___Spectrophotometer____ also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at ____260nm______ and using the following equation: Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml___ When extrapolated from a standard curve, the Bradford data indicates the amount (in _ug__ ) of __unknown ___ protein found in a sample. If you know the volume of sample that was added to the assay then you can calculate the protein ____concentration ___ of the sample. The coomassie blue dye in the Bradford assay specifically binds primary ¬¬___sulfonic___ and __positive amines__ groups of the amino acid side groups of the proteins. The more the dye binds to the sample, the _anionic_ the blue color will be, and the absorbance at 595¬nm will be_shifted Amax___. A sample with an unusually __protein___ number of ___280nm of Tyrosine __ [give a specific example] amino acids will underestimate the total amount of protein present in the sample.
In the case of the gene that determines high cholesterol in the blood, the two alleles express incomplete dominance.
What this means is that the dominant allele is not completely dominant over the recessive allele. If the allele was completely dominant, even one allele would be enough to determine the individual's trait as dominant. But in the case of incomplete dominance between the alleles, the heterozygous individuals that have one dominant and one recessive allele are an ''in between'' phenotype.
Resulting factors are called Second-order factors
<h3>
What is factor analysis?</h3>
- Factor analysis is a statistical approach for describing variability in seen, correlated variables in terms of a possibly smaller number of unobserved variables known as factors.
- It is possible, for example, that fluctuations in six known variables mostly reflect variations in two unseen (underlying) variables.
- Factor analysis looks for such joint fluctuations in response to latent variables that are not noticed.
- Factor analysis may be regarded of as a specific form of errors-in-variables models since the observed variables are described as linear combinations of the possible factors plus "error" terms.
- It may help to deal with data sets where there are large numbers of observed variables that are thought to reflect a smaller number of underlying/latent variables.
- It is one of the most commonly used inter-dependency techniques and is used when the relevant set of variables shows a systematic inter-dependence and the objective is to find out the latent factors that create a commonality.
To Learn more about factor analysis from the given link
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Answer:
Mendel was an Austrian monk whose researches laid the foundation of genetics. The experiments conducted by Mendel led him to the foundation of two laws which are named as the law of segregation and law of independent assortment.
According to the law of segregation, the two alleles of a gene segregate during the time of gamete formation and there are 50-50% chances of each of the alleles to be received by the gametes. Hence, there are 50% chances for Y gametes to be produced and 50% chances for y gametes to be produced.
I believe the answer is the responding stage; which is the fourth stage of listening whee one sues feedback to demonstrate to the speaker their thoughts. Feedback is used in this stage and may be verbal or non verbal. The positive feedback; while the speaker is talking, might include, alert posture, direct eye contact, appropriate facial expressions, head nods and supportive phrases such as "go on" and 'uh-huh". After speaker talks might include requests for clarification or emphatic statements.
