B. They compare traits that species have
Termite, vector, or parasites
Overproduction
Most species produce more offspring than can survive.
Competition
Since living space and food are limited, organisms must compete for the necessities.
Variation
Differences between individuals in a population.
Adaptations
Any kind of inherited trait that improves an organism's chances of survival.
Natural selection
The environment selects organisms with optimal traits to be the parents of the next generation.
Speciation
Over many generations, favorable adaptations accumulate and may result in a new species.
some gets converted into sound energy and escapes into the surroundings.
hope it helps..!!!
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
