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Alisiya [41]
3 years ago
8

When cloning eukaryotic genes into bacteria for protein production, a copy of DNA that lacks introns must first be obtained. Int

rons are removed from primary RNA transcripts by the cell's own machinery, but the copy of the DNA lacking introns must be generated by a biotechnology method. This intron-free copy DNA (or cDNA) is generated by
Biology
1 answer:
Paladinen [302]3 years ago
6 0
Obtaining mRNA from the cell, then using reverse transcriptase enzyme to convert it into a stable cDNA (or copy of DNA) molecule for insertion into the bacterial cell.










I hope this helps!
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In sickle-cell disease, malformation of red blood cells is caused by only one mistake for one amino acid in the genetic code for
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Answer:

A. True

Explanation:

Hemoglobin is a protein and has two distinct types of polypeptide chains. These are called the alpha and beta subunits. The gene that code for the beta chain undergoes a mutation in a single base that causes sickle cell anemia. Here, adenine base in the genetic code for glutamic acid is substituted with a thymine base.

The genetic code for glutamic acid in the beta chain gene is GAG. The "A" is replaced with "T" and the new code "GTG" codes for valine. Therefore, the mutated hemoglobin has valine in place of glutamic acid. This makes these mutated protein molecules to form aggregates resulting in a change in the shape of RBCs carrying them.

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3 years ago
Describe a stoma, and relate the function of the stoma to your description.
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Answer:

BOTONY:

any of the minute pores in the epidermis of the leaf or stem of a plant, forming a slit of variable width which allows movement of gases in and out of the intercellular spaces.

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an artificial opening made into a hollow organ, especially one on the surface of the body leading to the gut or trachea.

Explanation:

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3 years ago
A young animal has never had much energy. He is brought to a veterinarian for help and is sent to the animal hospital for some t
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Answer:

A. His mitochondria lack the transport protein that moves pyruvate across the outer mitochondrial membrane.

Explanation:

Pyruvate is from the breakdown of carbohydrates such as glucose through glycolysis. Glucose enters the cytosol through specific transporters (the GLUT family) and is processed by one of several pathways depending on cellular requirements. Glycolysis occurs in the cytosol and produces a limited amount of ATP, but the end product is two 3-carbon molecules of pyruvate, which maybe diverted again into many pathways depending on the requirements of the cell. In aerobic conditions, pyruvate is primarily transported into the mitochondrial matrix and converted to acetyl-coenzyme A (acetyl-CoA) and carbon dioxide by the pyruvate dehydrogenase complex (PDC).

Initially it was proposed that pyruvate was able to cross the membrane in its undissociated (acid) form but evaluation of its biochemical properties show that it is largely in its ionic form within the cell and should therefore require a transporter.

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3 years ago
Cooking oil and gasoline (a hydrocarbon) have no affinity for water because they _____.
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Answer;

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A simple example is that lipids (fat) can not circulate the body along with blood unless it is bonded to an amphipathic molecules or else lipids will not be able to move in the vessels and will make obstructions.

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3 years ago
Please answer this question!!!
Tanzania [10]

the answer your looking for :

A. genus and species

hope this helps!!

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