Question

When cloning eukaryotic genes into bacteria for protein production, a copy of DNA that lacks introns must first be obtained. Introns are removed from primary RNA transcripts by the cell's own machinery, but the copy of the DNA lacking introns must be generated by a biotechnology method. This intron-free copy DNA (or cDNA) is generated by

Answer 1

Obtaining mRNA from the cell, then using reverse transcriptase enzyme to convert it into a stable cDNA (or copy of DNA) molecule for insertion into the bacterial cell.










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