Answer:
Please look at the explanation section.
Explanation:
1) To calculate the yield of a protein in the purification step, the amount of protein produced must be divided by the amount of protein produced in the first step. The expression is as follows:
%protein yield = (Amount of protein/Amount of protein produced in the first step) * 100
If you have the purity percentage and the amount of protein produced, this formula could be used:
%protein yield = (Protein amount * Purity Percentage)/100
2) If this process begins with a cell culture and ends with the purification of Ni+2NTA. One method of increasing performance is to decrease the number of steps in the purification process. Because unfortunately, at each step of the purification of a protein leads to the loss of them.
Answer: Protein molecules are made through the transcription of a DNA strand into an mRNA strand and the translation of the mRNA into a protein. Transcription takes place in the nucleus of the cell while translation takes place in the ribosome.
Explanation: Transcription is a process of copying the genetic message encoded in a DNA strand into a messenger RNA (mRNA). This process is the first step in protein synthesis and takes place in the nucleus. It is catalyzed by an enzyme known as RNA polymerase. The synthesized mRNA then leaves the nucleus and enters the ribosome where translation occurs. During translation, the mRNA passes through the ribosome complex three nucleotides at a time where the codon is read and the corresponding amino acid coded for is fetched and attached to the peptide chain by the transfer RNA (tRNA). Translation starts when the ribosome encounters the start or initiation codon on the mRNA molecule. The start codon is AUG and it codes for methionine. Translation stops when the ribosome encounters the stop or termination codon. There are three termination codons and they are so named because they do not code for any known amino acid. The termination codons are UAG, UAA and UGA.
Hey there! I believe the answer is the fourth option: D. Four haploid cells
Hope this helps! :)