Answer:
Since high ethanol is a major stress during ethanol fermentation, ethanol-tolerant yeast strains are highly desirable for ethanol production on an industrial scale. A technology called global transcriptional machinery engineering (gTME), which exploits a mutant SPT15 library that encodes the TATA-binding protein of Saccharomyces cerevisiae (Alper et al., 2006; Science 314: 1565-1568), appears to be a powerful tool. to create ethanol tolerant strains. However, the ability of the strains created to tolerate high ethanol content in rich media remains to be demonstrated. In this study, a similar strategy was used to obtain five strains with higher ethanol tolerance (ETS1-5) of S. cerevisiae. When comparing the global transcriptional profiles of two selected strains ETS2 and ETS3 with that of the control, 42 genes that were commonly regulated with a double change were identified. Of the 34 deletion mutants available in an inactivated gene library, 18 were sensitive to ethanol, suggesting that these genes were closely associated with tolerance to ethanol.
Explanation:
Eight of them were novel and most were functionally unknown. To establish a basis for future industrial applications, the iETS2 and iETS3 strains were created by integrating the SPT15 mutant alleles of ETS2 and ETS3 into the chromosomes, which also exhibited increased tolerance to ethanol and survival after ethanol shock in a rich medium. Fermentation with 20% glucose for 24 h in a bioreactor revealed that iETS2 and iETS3 grew better and produced approximately 25% more ethanol than a control strain. The performance and productivity of ethanol also improved substantially: 0.31 g / g and 2.6 g / L / h, respectively, for the control and 0.39 g / g and 3.2 g / L / h, respectively, for iETS2 and iETS3.
Therefore, our study demonstrates the utility of gTME in generating strains with increased tolerance to ethanol that resulted in increased ethanol production. Strains with increased tolerance to other stresses such as heat, fermentation inhibitors, osmotic pressure, etc., can be further created using gTME.
Cell organelles are located in the Cytoplasm of the cell
The answer is C: because growth at 37°C would be ideal for revealing bacteria that are human pathogens. 37°C is equivalent to 98.6°F, the normal body temperature for humans. If bacteria are reproducing at this temperature in a petri dish, they are also most likely reproducing in the body.
Cultures are made so doctors can be sure a person is sick with a specific bacteria often in order to make sure they are taking the right medication to get better. Choice A doesn't make sense, because we wouldn't want to kill the bacteria we are trying to study. Bacteria that makes us sick is harmful bacteria and is what we are trying to isolate. Choice B doesn't make sense, because they are only being incubated at one temperature, not a range or variety. Choice D is harder to rule out, but again the doctor wants the bacteria to reproduce so they can be sure that's what is causing the infection, so it wouldn't make sense that we would put the bacteria in a temperature they would not reproduce.
Answer:
I'll inform them that the possibility of all their future children/offspring being phenotypically sickle-celled is very high.
Explanation:
Sickle cell is an inherited disease condition in which the red blood cells of the blood loses its shape and hence, dies or gets broken down. It has to do with the blood genotype of an individual. There are three major types of blood genotypes in humans namely: AA, AS, and SS. SS is the recessive genotype that codes for the sickle cell trait.
Hence, a human with the sickle cell trait has a genotype- SS. Therefore, according to this question, a man and a woman, each with sickle-cell trait (SS), were planning to marry, This will mean that both the man and the woman will always produce a gamete with S allele, which will combine to form an SS offspring. In other words, all of the offsprings of this man and woman will be sickle-celled.
