EDTA inhibits the activity of DNase during DNA isolation process.
DNase is an enzyme which degrades DNA using divalent metal ions like Mg^{2+} . These metal ions act as cofactor for the proper functioning of DNase.
EDTA chelates the divalent metal ions and thus does not allow DNase to access them.
Therefore in this way DNA is prevented from degradation during its isolation.
<span>If a population of medium sized rodents is placed under a selective pressure that yields both very large and very small individuals it was most likely directional selection. I hope that this is the answer that you were looking for and it has actually come to your desired help.</span>
A microscope because it needs to be magnified
Answer:
The correct answer would be 5' capping, 3' polyadenylation , and intron splicing.
In eukaryotes, transcription results in the formation of pre-mRNA which needs to be processed in order to become mRNA (messenger ribonucleotide).
The pre-mRNA processing include:
- Addition of 5'cap (usually modified guanine nucleotide) at the 5'-end of the RNA. It prevents the RNA degradation and also help in the attachment of ribosome for translation.
- Addition poly-adenine (poly-A) tail at the 3'-end of the RNA. It increases the stability of the RNA and helps in its export out of the nucleus.
- Lastly, splicing takes place which removes the introns and joins the exons. Alternative splicing can also take place in order to produce many mRNA from a single gene.
