Answer:
Gram staining procedure is used to differentiate between the gram-positive and gram-negative bacteria. various reagents used in the gram staining are crystal-violet, iodine, ethanol, and safranin.
1. First, the primary strain that is crystal violet is used to stain the bacterial cells present present on the slide.
2. Then the iodine solution is added over the bacterial culture and held for 1 minute. Iodine makes a complex with the crystal violet in the cell wall of bacteria.
3. Then acetone is used as a decolorizer that decolorizes the gram-negative bacteria by dissolving its lipopolysaccharide cell wall but do not decolorize gram-positive bacteria because its cell wall is made up of peptidoglycan layer.
4. The last reagent is safranin which is used to stain decolorized gram-negative cells pink. Therefore the correct order of staining is:
Crystal violet→Iodine→acetone→sefranine
The whale worms feed on the bones of the whale. The skeleton of the whale consists of huge amount of oil and thus is able to support enormous life. Usually a large whale fish bone is made up of 60% oil which is approximately equal to 6-7 tons of oils. The male whale worms live with in the bodies of the female worms. As many as 50-100 males may reside in one female. These worm species live in dandelion weeds and produce lots of eggs, grow fast and then disperse fast.
After consuming whale skeleton, all the worms at that site will die off. Before dying they release lots of eggs that are transported by the ocean currents and survive until they find whale bones to colonize
The correct answer would be A or the first option
Boundary between two air masses of different temperatures and/or humidity levels
