Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Answer:
The suitable option will be - B
Citric acid cycle
Explanation:
The results were -
Glucose: None
CO2: Increase
O2: None
ATP: Increase
NADH: Increase
- We know that the Citric acid cycle is also known as the TCA cycle (tricarboxylic acid cycle) or the Krebs cycle. This cycle occurs in Mitochondria in case of eukaryotes. In this cycle acetyl-CoA is oxidized which comes from carbohydrates, proteins and fats and energy is released in the form of ATP.
- water is also used in this process. NAD+ gets reduced to NADPH. In this system carbon dioxide is produced as a waste byproduct. ATP is produced as the main product. ATP is also used as a source of energy.
It is A, the cell is divided into a parent cell and daughter cell (mitosis)
Answer:
The steps involves making hypothesis, deriving predictions from the logical consequences and then carrying out experiments based on those predictions.
Explanation:
Step 1: make a observation .
Step 2: Formulate a statement or question.
Step 3: Form the hypothesis .
Step 4: make a prediction based on the hypothesis.
Step 5: test the hypothesis whether its true or not.
Step 6: use the results to conclude the experiment.
Answer:
In terms of adaptation, fitness refers to the ability of an organism to survive and reproduce in a constantly changing environment.
Adaptive traits are very much important in a constantly changing environment as they increase the chances of survival of a population in the environment.
For example, Darwin's finches adapted to develop different types of beaks in order to get nutrition in different environments. Long necks of giraffes are also considered as a result of adaptation which helps them get the food located high on trees.
