Answer:
The wolf population has substantially declined in Canada.
Explanation:
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The correct order of steps in a gram staining procedure is:
(4) the specimen is treated with a stain called crystal violet.
(1) iodine is added as a fixative or mordant.
(3) the specimen is flushed with alcohol to dehydrate peptidoglycans and trap the stain.
(2) the specimen is treated with a stain such as safranin or fuchsin (a pink stain)
Gram staining is a common staining technique which helps to classify the bacteria into two categories: Gram positive and Gram negative. Gram positive bacteria appear violet in color and Gram negative bacteria appear to be pink. This color difference appears due to the difference in the bacterial cell walls.
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Similarly, our surroundings are our environment. The social and economic situation of a person is influenced by all environmental influences. It results in progress or positive change in the society and nation's social, economic, and cultural components. Peace and security are brought about by development.
