Answer:
knowledge of the sequence product
Explanation:
A gene knockout is a technique used in molecular genetics to deactivate target genes in an organism in order to study their functions by reverse genetics (i.e., gene loss). Knockouts are generated by different methods including, for example, homologous recombination or site-specific nucleases (zinc-fingers, TALENS, CRISPR/Cas9). These techniques require to know a priori the sequence of each gene to be knocked out in order to target desired mutations. In the last years, the CRISPR/Cas9 tool has gained attention to knockout genes of interest because it is a genome editing system that can be easily used for deletion or insertion of bases.
<span>When a complete set (genome) of chromosome is added or subtracted the condition is called Euploidy. When there is addition or deletion of a single member chromosome the condition is called Aneuploidy.</span>
There are microorganisms that are able to live in extreme environments under adverse conditions of pH, temperature and salinity. These microorganisms are classified as extremophiles. Within the group of extremophiles there are halophilic bacteria, which are those capable of living in extremely saline environments.
One biological factor that all living things are subject to suffer from is osmotic pressure. Halophilic microorganisms have developed mechanisms to adapt to saline environments where osmotic pressure acts with great intensity on individuals. These bacteria change the chemical composition of their membranes and also accumulate osmoprotective compounds in their cytoplasm to compensate for osmotic stress.
RAMIREZ, N; SANDOVAL, AH y SERRANO, JA. Las bacterias halófilas y sus aplicaciones biotecnológicas. Rev. Soc. Ven. Microbiol. [online]. 2004, vol.24, n.1-2 [citado 2019-09-22], pp. 12-23 . Disponible en: <http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S1315-25562004000100004&lng=es&nrm=iso>. ISSN 1315-2556.
