The restriction endonuclease EcoRI cleaves the DNA sequence 5'-GAATTC-3' in such a way as to leave a 5' overhang, which is often referred to as a "sticky end". To 5' overhang with the complementary sequence this sticky end adhere.
- The palindrome EcoRI restriction site breaks DNA after G and creates sticky ends with AATT. G/corresponding AATTC's DNA sequence is CTTAA/G, where "/" designates the location of the peptide bond break.
- When the ssDNA sequence 5'-GAATTC'-3 is recognised by the restriction endonuclease EcoRI, a single-strand cut is made between the G and A nucleotides.
- At the ultimate end of double-stranded DNA, an overhang is single-stranded DNA. This can occur on either strand, and if it does, there will either be a 5' overhang on the 5' end of the strand or a 3' overhang on the 3' end of the strand.
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Answer:
The five bases are adenine, guanine, cytosine, thymine, and uracil, which have the symbols A, G, C, T, and U, respectively. The name of the base is generally used as the name of the nucleotide, although this is technically incorrect.
Explanation:
Answer:
Complete questions include: Answer Bank: Guanine nucleotides, uridine nucleotides, adenine nucleotides, cytosine nucleotides.
Explanation:
Glycine Auxotroph: Guanine nucleotides and adenine nucleotides (Purine nucleotides)
Glutamine auxotroph: Guanine nucleotides and adenine nucleotides (purine nucleotides)
Aspartate auxotroph: Guanine nucleotides, uridine nucleotides, adenine nucleotides, cytosine nucleotides Both purine and pyrimidine nucleotides)
The amino acids glutamine, glycine, and aspartate provide all the nitrogen atoms of purines in a two ring-closure steps to form the purine nucleus. Pyrimidines are synthesized from carbamoyl phosphate and aspartate.
