Answer: "To move substances against a concentration or electrochemical gradient, a cell must use energy. Active transport mechanisms do just this, expending energy (often in the form of ATP) to maintain the right concentrations of ions and molecules in living cells."
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All raw meat contains bacteria that can be harmful to our bodies. Different types of meat have different temperatures to which we have to cook it to kill this bacteria, so we can eat it without getting sick.
Well assuming the original cell is a prokaryotic, you wouldn't be able to remove the nucleus because prokaryotic cells don't have a nucleus to begin with. if this doesn't help then: The cell would lack genetic information.