Answer:
Each mutant would be mated to wild type and to every other mutant to create diploid strains. The diploids would be assayed for growth at permissive and restrictive temperature. Diploids formed by mating a mutant to a wild type that can grow at restrictive temperatures identify the mutation as recessive. Only recessive mutations can be studied using complementation analysis. Diploids formed by mating two recessive mutants identify mutations in the same gene if the diploid cannot grow at restrictive temperature (non-complementation), and they identify mutations in different genes if the diploids can grow at restrictive temperature (complementation).
Explanation:
Recessive mutations are those whose phenotypic effects are only visible in homo-zygous individuals. Moreover, a complementation test is a genetic technique used to determine if two different mutations associated with a phenotype colocalize in the same <em>locus</em> (i.e., they are alleles of the same gene) or affect two different <em>loci</em>. In diploid (2n) organisms, this test is performed by crossing two homo-zygous recessive mutants and then observing whether offspring have the wild-type phenotype. When two different recessive mutations localize in different <em>loci</em>, they can be considered as 'complementary' since the heterozygote condition may rescue the function lost in homo-zygous recessive mutants. In consequence, when two recessive mutations are combined in the same genetic background (i.e., in the same individual) and they produce the same phenotype, it is possible to determine that both mutations are alleles of the same gene/<em>locus</em>.
Answer:
Explanation:
Long-term Self-Renewal: Stem cells are capable of division while remaining unspecialized.
Differentiation
: Stem cells can divide asymmetrically, where one stem cell divides into two cells that have different cellular fates.
Induced Pluripotency
: Stem cells that have been artificially created from specialized cells are called induced pluripotent stem cells.
Considerations
: Due to their unspecialized nature, stem cells have a large research potential. The most pluripotent stem cells derive from the human embryo.
Hello!
The two statements that disprove the theory of spontaneous generation are that A) All living things are made of cells and that D) Living cells can only come from previously existing cells.
The scientist that disproved the spontaneous generation theory was Louis Pasteur, who boiled a culture broth free of microorganisms and let it stand for a long period of time. No organisms grew from the flask, but when Pasteur opened it to the environment the flask soon became full of bacteria. That proved that there were microorganisms that were responsible for the growing of living things and disproved the theory of Spontaneous generation.
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