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3 years ago

Based on your understanding of nucleic acids, what type of bonds form between the CRISPR/guide RNA molecule and the target DNA

Biology

1 answer:

Tanya [424]3 years ago

6 0

Answer:

The single guide RNA forms hydrogen bonds with DNA, while Cas9 hydrolyzes phosphodiester bonds

Explanation:

The base pairing between nucleic acid strands (either DNA or RNA) is through hydrogen bonds between nucleotide bases. In DNA, Adenine always forms two hydrogen bonds with Thymine, while Guanine always forms three hydrogen bonds only with Cytosine. Moreover, adjacent nucleotides in the same strand are covalently linked by phosphodiester bonds (i.e., covalent bonds between the 5' phosphate group of one nucleotide and the 3'-OH group of another). The CRISPR/Cas9 genome editing systems make use of single-guide RNAs (sgRNAs) that interact with DNA through hydrogen bonds. These sgRNAs have perfect complementarity to the target DNAs in order to bind them. On the other hand, Cas9 is an enzyme that hydrolyzes phosphodiester bonds in both DNA strands very precisely and accurately by using a sgRNA complementary to a specific DNA sequence.

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Sophie [7]

Answer:

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3 years ago

Asexual reproduction creates offspring ______ the parent.

german

Asexual reproduction creates offspring that are identical to the parent.

4 0

3 years ago

The ABO blood group assorts independently of the Rhesus (Rh) blood group and the MN blood group. Three alleles, IA , IB , and i,

Eva8 [605]

Answer:

Part 1:

The mother’s genotype is ii, rr, MN

The father’s genotype is IAIA/IAi, Rr, MM

Part 2:

Approximately 1/32 (3.125%) of children can be expected to have the stated blood types.

Explanation:

Part 1:

The mother has blood types 0, Rh- and MN. Therefore, her genotype has to be ii, rr, MN.

The father has ABO type A. His ABO genotype may therefore be either IAIA or IAi, as both are compatible with type A offspring with a type 0 mother. His Rh genotype must be rr, since his Rh- child necessarily has to have inherited one r allele from each parent. Finally, his MN genotype has to be MM since both alleles are codominant.

Part 2:

Using the given data, we build the following Punnett chart:

IA R N IA r N i R N i r N

IA R M

IA R N

IA r M

IA r N

IB R M

IB R N

IB r M X

IB r N

Note that, of all 32 possible combinations, only a single one of them (marked with “X” produces a genotype which matches the desired blood type, as follows:

genotype: IBi, rr, MN

blood type: B, Rh-, MN

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3 years ago

Which of the following statements is false? The hormone progesterone causes the endometrium to become thicker. After the ovarian

bazaltina [42]

Answer:

A woman creates new immature ova in the ovary each time she has a menstrual cycle.

Explanation:

During the menstrual cycle, hormones present in the body make the immature eggs 'mature' ones, in the ovary. About in half of the cycle, hormones make the release of the mature egg from the ovary and travels towards uterus. It can remain there for 24 hours and if not fertilized the it breaks down and released out.

3 0

3 years ago

Read 2 more answers

(20 points) Click on the statement. Decide if it is true or false, then select that choice to add it to the appropriate set.

vaieri [72.5K]

The correct answer is I’m just doing this for points shehdheh ye

7 0

3 years ago