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The major contribution made by Avery, MacLeod, and McCarty was 'transformation of nonvirulent cells with a DNA extract was blocked only if DNase was added'. This observation supported the idea that the transforming agent in Griffith's experiment was DNA.
The Avery–MacLeod–McCarty experiment showed that DNA was the transforming agent observed in Griffith's experiment.
Griffith's experiment (1928) suggested that bacteria were capable of transferring genetic information (DNA) by a process of horizontal gene transfer called transformation.
While the bacteria were killed by heat, Frederick Griffith observed that the DNA had was taken up by II-R strain bacteria via transformation.
Avery–MacLeod–McCarty experiment observed that protease enzymes did not destroy the transforming agent (but DNAse destroyed this transforming principle), thereby suggesting that the transforming agent in Griffith's experiment was bacterial DNA.
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Answer: There are two factors that determine the urine flow rate during an osmotic diuresis: the rate of excretion of effective osmoles in the urine and the effective osmolality of the interstitial compartment of the renal medulla.
The normal range for 24-hour urine volume is 800 to 2,000 milliliters per day (with a normal fluid intake of about 2 liters per day).