To increase the novelty of Life.
The study in a Pennsylvania forest reveal about the environmental effects of using DDT reveals that a large number of ladybird were killed due to DDT which caused rise in the population of aphids.
DDT is a chemical that was used for killing the insects and keeping the crops safe and productive. It was later found that it has a worse effect on the lives of other non target organisms like other insects, bird and human as well.
It is also known to be associated with the phenomenon of biomagnification. It is a process of accumulation of harmful chemicals with increase in the trophic level which occurs due to continuous intake of food contaminated with chemicals that is not tolerable and causes serious implication.
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Part 1) 54 - 36 = 18
Part 2) You would just subtract 54 and 36 to get your answer because is says what is the DIFFERENCE meaning subtraction