True. Having only one trial means there is nothing to compare the results to and there could be multiple outliers within the data
Answer is D. Nitrogen fixation.
The root nodules of some plants contains Rhizobium bacteria which fixes atmospheric nitrogen into soil as Ammonia. Ammonia is converted into nitrate which is the most usable form of nitrogen in the soil. Nitrate is absorbed by the roots of the plants, from plants nitrogen reaches to animals when they eat plants.
Penicillin, then amoxicillin.