Frozen red blood cells that have been thawed, deglycerolized and reconstituted in an open system must be used within 24 hours.
Cryopreservation of Red blood cells (RBCs) can be done with a long time span of usability of 10 years. Glycerol safeguards RBCs during freezing and defrosting, yet it can cause hemolysis if it is not washed off or eliminated before transfusion. When a unit has been deglycerolized, the shelf life of RBCs in an open system is only 24 hours, bringing about sporadic utilization of Frozen RBCs from the outset of the procedure or in close system cases it is up to 14 days.
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No they do not since whilst the parents may exhibit a certain trait this does not mean that they are necessarily homozygous for that trait, and thus if for example we had two heterozygous individuals who had brown eyes, with B denoting brown eyes as the dominant trait and b denoting blue eyes as the recessive trait, their offSpring could have the genotypes BB, Bb or bb - we can see here that whilst the chance of having brown eyes is higher, the offspring may have either brown or blue eyes.
Answer:
b. False
Explanation:
These components are not sufficient to successfully perform a PCR reaction. Deoxynucleoside triphosphates (dATP, dGTP, dCTP, dTTP) are missing so that the PCR reaction can occur perfectly.
The PCR technique allows a specific fragment of the DNA molecule to be amplified thousands of times in just a few hours. This technique revolutionized research in molecular biology because it had taken a long time for DNA amplification. From PCR it is possible to obtain enough copies of a part of DNA to detect and analyze the sequence that is the target of the study.
For a PCR reaction to be performed a solution with some components must be prepared. These components are:
- Magnesium chloride buffer (to optimize reaction and act as a cofactor for polymerase)
- Forward and reverse primers (to customize the start of the enzyme reaction)
- DNA polymerase (Enzyme required for replication of desired DNA region.)
- DNA Template (the DNA to be copied)
- PCR-grade water
- Deoxynucleoside triphosphates: dATP, dGTP, dCTP, dTTP (act as bricks in the construction of DNA molecules).
