Question

One advantage of allele-specific oligonucleotide (aso)-based over rflp-based detection of human genetic disease is that _______.

Answer 1

An allele-specific oligonucleotide (ASO) is a tiny piece of artificial DNA balancing to the order of an adjustable target DNA. It performs as an enquiry for the occurrence of the target in a Southern blot assay or Dot blot assay. One advantage is the mutation need not be positioned within a limit enzyme recognition site.