1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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Answer:
true because of statute of limitations
Explanation:
Statute of limitations -
It is the law , according to which it decides the maximum number of times the party have to initiate the legal proceedings , is known as the statute of limitations .
The number of times , depends on the offense severity .
In case of very serious offence like murder , there are no maximum period of time .
Hence from the question , Callie's statement is true , due to statute of limitations .
Answer:
d) Outside factors that we cannot predict or control often impact the forecast.
Explanation:
Company or organization forecasting is a method or technique to predict the company's future. It is the technique to predict or estimate the future aspects of the company's business or may be other operations. Planning and predicting for the company for the future is a critical thing. Many factors influence the forecasting of an organization but the some outside factors make it difficult to predict the company's forecast.
Hence the correct option is (d).
Answer:
the benefits of Independence is you take care RB self and try not 2 try not to start a fire another reason is trying to prove yourself to your parents a risk is talking to strangers getting the cars with strangers another reason is you might hurt yourself by a fire are car accidents or getting a car or a car hitting you
Answer:
Option A: Identity Diffusion
Explanation:
diffusion in identity define as ways in which someone tries to understand or know themselves more.in adolescence stage, social identity, personality of a person are not usually or really known(part of self Discovery), and much work effort is not being utilized and put into to discover one's potential or what what wants from life and to do with life.
Most people or individuals in this state or stage are not really bothered about the future or what they want to be. It is a work in progress as people are gradually working on their identity building. The characteristics of individuals in this stage is that they are passive, not worried or bothered about the future, only consider the moment and usually less responsible in their life actions.
