Answer:
In gram staining, 95% ethanol is used to destain the gram-negative bacteria but gram-positive bacteria do not get destain because peptidoglycan layer in gram-positive cell wall does not allow crystal violet- iodine complex to come out of the cell wall.
Gram-negative bacteria do not have peptidoglycan cell wall and so crystal violet comes out of the cell wall by using 95% ethanol because ethanol dissolves the lipopolysaccharide membrane of gram-negative bacteria and allow the dye to leak out.
Therefore if I forgot to apply 95% ethanol than crystal violet dye will not come out of the lipopolysaccharide cell wall of gram-negative bacteria and it will appear violet under the microscope.
Gram negative and Gram positive refers to if bacteria retain the Gram Stain or not. Gram positive do. Gram negative do not. Gram positive bacteria will appear purple/blueish after staining. <span>It is important because whether a bacteria is Gram positive or negative helps determine the species, characteristics of disease, and the best antibiotics to use in treatment. </span>
False because <span>Like DNA, RNA is assembled as a chain of </span>nucleotides<span>, but unlike DNA it is more often found in nature as a single-strand folded onto itself, rather than a paired double-strand. Cellular organisms use </span>messenger RNA<span> (</span>mRNA<span>) to convey genetic information.</span>
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