Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
Genotype: Bb (heterozygous)
Phenotype: brown fur
Explanation:
Since allele B is dominant to allele b, as long as genotype has allele B in it, its effect of phenotype will be expressed instead, regardless of whether allele b is present.
As mouse genotype is stated as Bb, on the chromosome from its parents, allele B comes from one parent while allele b is inherited from the other parent.
Phenotype is the expressed trait. In this case, as genotype has allele B, the phenotype will be brown fur as its effect is expressed.
Question
Microarrays allow for
•a comparison of expression of different genes under different growth conditions.
• the analysis of global gene expression.
•the hybridization of cDNA (converted from mRNA with DNA affixed to a solid-state support.
• all of the above.
Answer:
• all of the above.
Explanation:
Microarray involves the collection and isolation of genetic samples (DNA) from a sample or tissue.
Microarray is composed of a large number of DNA material that is arrange sequentially for easy identification and use.
Microarray is used to compare the expressions of various types of genes that are growing under different conditions. It can also be used for analysis of genes globally.
Answer:
one normal girl
One normal boy
One carrier girl
One colour blind boy
Explanation:
Haemophilia is a sex linked feature as it is transmitted with the chromosomes determining sex.
In humans,the male has XY and the female has XX.the Y chromosome is usually genetically empty.so as mall received his Y chromosomes from his father,he cannot inherit his father's sex linked traits .but women can as they receive and X chromosomes from their fathers.so a cross between a carrier woman Cc and a normal man C result in;
XX--CC
XX--Cc
XY--C
XY--c
Answer:
the correct order of events in the nutritional processing of this food is ingestion- digestion- absorption- egestion.
Explanation:
1.) Ingestion involves taking food, drink, or another substance into the body by swallowing to aid in digestion.
2.) Digestion involves the breakdown of large insoluble food molecules into small water-soluble food molecules so that they can be absorbed into the watery blood plasma.
3.)Absorption involves the in take of the digested food into the bloodstream
4.) Egestion involves the removal of undigested food substance from the body.
