During the isolation, they managed to create a distinct cultural identity and a society that reflected their values because they did not have anything from foreign lands to affect their beliefs. They eventually met economic problems which led to the end of the isolation period and the country entered the Meiji restoration.
Some would fight off soldiers to flee and sacrifice themselves so other Natives from their tribe could stay. The Seminole refused to leave and started a war called the Second Seminole War. Another war caused by refusual to leave was the Black Hawk War.
B. I believe it was Puerto Rico. I just did an essay on Puerto Rico last semester.