Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
Leukemia may be defined as the cancer of the blood cells especially white blood cells. The leukemia may occur due to mutation or may also occur due to the biological agents.
Leukemia causes the abnormal and uncontrolled growth of the blood cells. These white blood cells are unable to fight with infections and causes the bone marrow to produce impair formation of blood cells. The patient shows abnormal growth and large count of leukocytes.
The monomer of carbohydrates, monosaccharides.
To prevent the invasive species from entering texas freshwater ecosystem the following steps should be taken they are described below.
Explanation:
The invasive species are dangerous to natural resources because they destroy the natural resources and ecological system.
These invasive species are controlled by the biological control and mechanical control.
Thus to prevent the invasive species from entering texas freshwater ecosystem the following steps are taken they are
- Always clean and dry any equipment used in or around lakes or rivers after each use.
- Spray off your boat after you take it out of the water.
- Never dump aquarium or pet species into sewer system of waterways.
Answer:
In geology, aseismic creep or fault creep is measurable surface displacement along a fault in the absence of notable earthquakes. An aseismic creep exists along the Calaveras fault in Hollister, California.
Explanation:
:) hope this helps
