Either A. or D. it depends on what kind of insulation you're looking for.
The answer is Permian-Triassic extinction.
The Permian-Triassic extinction was 252 million years ago. It is known as The Great Dying because about 95% of all species died out due to environmental changes (volcanic eruptions, dry climate, melting of ice sheets, etc). This is also the only event in which many insect species wiped out.
In low light no plants grow. With no plants no animals can eat so less animals remain or stay there. With less animals less animals respire and breathe out CO2.
The question that could be investigated through scientific research is B. Does a newly synthesized chemical reduce bacteria? The answer is B. Because the other questions aren’t science questions. They are bias.