Answer:
By way of introduction, A260/A280 ratio is use to measure Protein Contamination. This procedure was first described to measure protein purity in the presence of nucleic acids. However it is now commonly used to assess protein contamination of DNA. The method to determine the concentration of protein contamination by using A260/280 ratio method is well explained below by filling in the missing words.
Explanation:
The A260/A280 ratio method estimates protein __purity___ by measuring the absorbance maximum at 280nm caused by the amino acids__cytosine___, ____Adenine____, and ___Guanine_________ Since ___Spectrophotometer____ also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at ____260nm______ and using the following equation: Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml___ When extrapolated from a standard curve, the Bradford data indicates the amount (in _ug__ ) of __unknown ___ protein found in a sample. If you know the volume of sample that was added to the assay then you can calculate the protein ____concentration ___ of the sample. The coomassie blue dye in the Bradford assay specifically binds primary ¬¬___sulfonic___ and __positive amines__ groups of the amino acid side groups of the proteins. The more the dye binds to the sample, the _anionic_ the blue color will be, and the absorbance at 595¬nm will be_shifted Amax___. A sample with an unusually __protein___ number of ___280nm of Tyrosine __ [give a specific example] amino acids will underestimate the total amount of protein present in the sample.
The cell would have to take in and use more energy in order to break the covalent bonds.
The correct option is B
Hydrogen bonds :
are the chemical mechanism that governs the complementarity of the bases of DNA. This correspondence is unique thanks to the geometry of the hydrogen donor atoms and the acceptors that form the bases.
The (hydrophobic) bases are stacked inside the double helix of DNA; their plane is perpendicular to the axis of the double helix. The outside (phosphate and sugar) is hydrophilic.
The hydrogen bonds between the bases of one strand and the bases of the other strand keep the 2 strands united.
One purine on one strand necessarily binds to a pyrimidine on the other strand. As a corollary, the number of purine residues is equal to the number of pyrimidine residues.
* A binds to T (by 2 hydrogen bonds).
* G binds to C (via 3 hydrogen bonds: more stable bond: 5.5 kcal vs 3.5 kcal).
What part of the DNA strand do hydrogen bonds hold together?
hydrogen. Covalent bonds occur within each linear strand and strongly bond the bases, sugars, and phosphate groups (both within each component and between components). Hydrogen bonds occur between the two strands and involve a base from one strand with a base from the second in complementary pairing.
Learn more about DNA strand:
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Answer:
The total amount of carbon is constant but limited as it moves through the carbon cycle.
Explanation:
