<span>Before answering the question, some explanation about the purpose of flow cytometry.
The cytometry analysis provides us with the presence and the abundance of </span><span>clusters of differentiation (CD), for example, the research of CD2, CD3 and CD4 in lymphocytes.
</span>These CDs will be marked (if they are present) with specific antibodies linked with different fluorochromes, and when the <span>cells exit the flow cell intersect, a laser detects these fluorochromes (representing CDs) and count them.
</span><span>The result will be displayed in a graph showing the frequency of the CDs with spots of different colours.
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True in most cases unless they erode then the younger ones will be on top
Answer:
d. DNA polymerase begins adding nucleotides at the 5' end of the template
Explanation:
Polymerase DNA always works in one direction, synthesizing the new 5' to 3' oriented chains and adding nucleotides to the 3' end of a new synthesis chain by forming phosphodiester bonds between the phosphate of a nucleotide and the sugar of the anterior nucleotide.
Because DNA polymerase only acts in a 5' to 3' direction, replication along a chain, the leading chain, occurs continuously. The synthesis of the opposite chain, the delayed chain, occurs discontinuously because the DNA polymerase must wait for the replication fork to open. Over the delayed chain, short segments of DNA called Okazaki fragments (named after Reiji and Tsuneko Okazaki, the scientists who discovered these fragments) are synthesized as polymerase DNA works out of the replication fork. Ligase DNA catalyzes the covalent bonds between Okazaki fragments in the delayed chain to ensure there are no gaps in the phosphodiester skeleton. Finally, the first ones are removed and these gaps are filled by the DNA polymerase.
Photosynthetic organisms use the process of photosynthesis to produce their energy. During this process they also take in carbon dioxide and release oxygen. If there were less of such organisms, there would be more carbon dioxide and less oxygen in the atmosphere.
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