Similar to the reaction that occurs in step 1 of glycolysis, a second molecule of ATP provides the phosphate group that is added on to the F6P molecule. The enzyme that catalyzes this reaction is phosphofructokinase (PFK).
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Microorganisms such as bacteria can be removed by disinfection. Certauns substances called biocides are addes to water to make it pure. Purification can also be done by UV-light .
