Bright cherry red color is produced upon the addition of methyl red indicator to a culture of a Gram negative bacilli in MR-VP broth if the organism used the mixed acid pathway of fermentation.
What is MR-VP test?
- As part of the IMViC testing, Methyl Red (MR) and Voges-Proskauer (VP) broth is utilized as the medium in which both the Methyl Red and Voges-Prosakuer tests can be conducted.
- It is a straightforward broth made up of dextrose or glucose, buffers, and peptone.
- In the Methyl Red test, an infected tube of MR-VP broth is mixed with the pH indicator methyl red.
- The acids will overwhelm the buffers in the medium and create an acidic environment if the organism employs the mixed acid fermentation pathway and creates stable acidic end products.
- If acidic end products are present when methyl red is introduced, the methyl red will remain red.
Learn more about the MR-VP test with the help of the given link:
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Answer:
All results for the experimental groups involving wing surgery would be invalid.
Explanation:
The experimental group involving wing surgery would not lead to correct results because it is a direct parameter that is impaired. This has been already clearly stated in the question that these flies are prone to attack compared to those which are not treated. There could be several reasons for that such as: (1) the reattachment would not have been successful, and/or (2) the flies' health (fitness) status would have been compromised.
On the other hand, it is not correct that all experimental groups will lead to incorrect results because the untreated flies were not attacked (or less attacked). Similarly, there is no condition given for houseflies rather only zonosemata flies. So 3rd option is also not possible. Finally, the reliability of the results would definitely change because the treated flies would be more attacked and lead to a clear difference in results.
