The matrix (inside mito) has a negative charge compared to the intermembrane space (outside of mito but still inside) because positively charged protons are constantly pumped from the matrix to the intermembrane space. A cell oxidizes an organic molecule producing 3 NADH molecules and 3 FADH2 molecules..
Eukaryotic organisms contain translocation factors that are suited to perform the same function. A translocation that results in Tu being replaced with G or G being replaced with Tu will not affect the function of the protein.
