Answer: (a) = 0.022mmoles/minute; (b) = 0.00022mmoles/minute; (c) = 0.022mmoles/minute
Explanation:
The Michaelis- Menten equation is used to show the relationship between substrate concentration and reaction rate.
The equation is Vo = Vmax[S]/(Km + [S]
where Vo is the initial velocity, Vmax is the maximum velocity, [S] is the substrate concentration, Km is the Michaelis constant.
(a) For enzyme 1; Vo = 0.002mmoles/minute, Vmax = ?, [S] = 0.1mM, Km =1.0mM; making Vmax subject of the formula;
Vmax = Vo * (Km + [S])/ [S]
Vmax = 0.002*(1.0 + 0.1)/ 0.1
Vmax = 0.022mmoles/minute (Vmax for Enzyme 1&2)
(b) Vo = Vmax[S]/(Km + [S]
Vo = 0.022 * 0.1/(10+0.1) = 0.00022mmoles/minute
(c) Vo = Vmax[S]/(Km + [S]
Vo = 0.022*1000/(1.0+1000) = 0.022mmoles/minute
