Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
False negative is a condition when you got a negative result for a specimen which was positive. To prevent false negative occur, first you need to use golden standard test or a test with high specificity. You can do multiple tests to ensure the specimen is negative and using a positive control to make sure the reagent is still working fine.
Answer:
In human males, meiosis occurs in the seminiferous tubules of the testicles while in females, it occurs in cells called as oogonia. In males, meiosis occurs at puberty while in females it occurs right at birth.
Explanation:
"was used by economist John Kenneth Galbraith to describe the prosperity of the 1950s" is the statement among the choices given in the question that is not <span>true of the phylogenetic tree. The correct option among all the options that are given in the question is the first option or option "a". I hope it helps you.
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