The burning of fossil fuels will affect the carbon cycle because it will put more carbon in the air, causing the carbon dioxide rate to increase and it will eventually lead to global warming
Answer:
Most liquids become monotonically denser when cooled from room temperature, but liquid water reaches its maximum density at approximately 4°C, below which it expands to become less dense as it is cooled further. To explain this density anomaly, several ideas have been proposed.
Explanation:
How many electrons the two atoms are sharing determines how many covalent bonds two atoms can make. :)
Answer:
Stay in the interior portion of your house, away from windows, skylights, and glass doors. Keep several flashlights and extra batteries handy. If your house is damaged by the storm you should turn the water and gas off at the main valves. i hoped thsi helped
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.