size hence streams are little and rivers are bigger than streams because a stream connects between two or more rivers
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The correct order of steps in a gram staining procedure is:
(4) the specimen is treated with a stain called crystal violet.
(1) iodine is added as a fixative or mordant.
(3) the specimen is flushed with alcohol to dehydrate peptidoglycans and trap the stain.
(2) the specimen is treated with a stain such as safranin or fuchsin (a pink stain)
Gram staining is a common staining technique which helps to classify the bacteria into two categories: Gram positive and Gram negative. Gram positive bacteria appear violet in color and Gram negative bacteria appear to be pink. This color difference appears due to the difference in the bacterial cell walls.
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We see that this is a region near the continents, so it is probable that there is the boundary of a tectonic plates around there. Since there are islands there, it is quite probable that there is a subduction zone near them which means that the oceanic crust is going below the continental one and the continental is slowly elevated. This also shows that the boundary is convergent, since transform boundaries do not lead to elevation. Near convergent boundaries, there are frequently volcanoes and shallow earthquakes. Finally, the climate near Alaska is cold and this does not depend on whether islands are near a boundary or not. So, 2 4 and 5 are correct.
