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Answer: B (GTP serves as the energy input for AMP synthesis from IMP)
Explanation:
In the de novo synthesis of purine nucleotide, IMP represents a branch point for purine biosynthesis. IMP can be converted to either AMP or GMP.
The formation of AMP from IMP requires GTP; similarly formation of GMP requires ATP. The conversion of IMP to either AMP or GMP is highly regulated – AMP and GMP inhibits their own formation by feedback inhibition of adenlyosuccinate synthetase and IMP dehydrogenase.
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The release of pyrophosphate from the incoming nucleotide, and then hydrolysis of the pyrophosphate to inorganic phosphate provides energy for the addition of nucleotide onto a DNA strand.
Nucleotides are linked together by a condensation event that yields a tiny, stable molecule. But the released molecule is pyrophosphate, not water. A good amount of free energy is released when water is added to pyrophosphate.
The high-energy link between the ejected beta and gamma phosphates stores the energy for each incoming nucleotide's addition. The subsequent hydrolysis that occurs drives the process. A substantially greater quantity of energy is released when two phosphates are separated into individual phosphates.
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